Single Molecules
Single Molecules |
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| 1 | Structure of DNA-cationic liposome complexes: DNA intercalation in multilamellar membranes in distinct interhelical packing regimes JO. Radler, I. Koltover, T. Salditt, CR. Safinya, Science (1997-02-07) 275-5301 p.810 Science publications Publisher : AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA. ISSN : 0036-8075 Abstract : Cationic liposomes complexed with DNA (CL-DNA) are promising synthetically based nonviral carriers of DNA vectors for gene therapy. The solution structure of CL-DNA complexes was probed on length scales from subnanometer to micrometer by synchrotron x-ray diffraction and optical microscopy. The addition of either linear lambda-phage or plasmid DNA to CLs resulted in an unexpected topological transition from liposomes to optically birefringent liquid-crystalline condensed globules. X-ray diffraction of the globules revealed a novel multilamellar structure with alternating lipid bilayer and DNA monolayers. The lambda-DNA chains form a one-dimensional lattice with distinct interhelical packing regimes. Remarkably, in the isoelectric point regime, the lambda-DNA interaxial spacing expands between 24.5 and 57.1 angstroms upon lipid dilution and is indicative of a long-range electrostatic-induced repulsion that is possibly enhanced by chain undulations. Corresponding Author : Affiliation(s) : (0) UNIV CALIF SANTA BARBARA,DEPT MAT,SANTA BARBARA,CA 93106.; (1) UNIV CALIF SANTA BARBARA,DEPT PHYS,SANTA BARBARA,CA 93106.; (2) UNIV CALIF SANTA BARBARA,BIOCHEM & MOL BIOL PROGRAM,SANTA BARBARA,CA 93106.; Key words : SYNCHROTRON X-RAY; GENE-THERAPY; PHASE; TRANSFECTION; EXPRESSION; HUMANS Type : Article, English. 1997-02-07 Time cited 433; Journal impact factor for year 1997 equals 24.676 [0] BEHR JP, 1994, BIOCONJUGATE CHEM, V5, P382 [1] BLOOMFIELD VA, 1991, BIOPOLYMERS, V31, P1471 [2] BOLTENHAGEN P, 1992, PHYS REV A, V46, P1743 [3] CAPLEN NJ, 1995, NAT MED, V1, P39 [4] CRYSTAL RG, 1995, SCIENCE, V270, P404 [5] DAN N, IN PRESS BIOPHYS J [6] EVANS EA, 1986, P NATL ACAD SCI USA, V83, P7132 [7] FELGNER PL, 1987, P NATL ACAD SCI USA, V84, P7413 [8] FELGNER PL, 1991, NATURE, V349, P351 [9] GERSHON H, 1993, BIOCHEMISTRY-US, V32, P7143 [10] GUSTAFSSON J, 1995, BIOCHIM BIOPHYS ACTA, V1235, P305 [11] HELFRICH W, 1978, Z NATURFORSCH A, V33, P305 [12] KAMIEN RD, 1996, PHYS REV E A, V53, P650 [13] KEMWORTHY AK, 1995, BIOPHYS J, V68, P1921 [14] KOLOVER I, UNPUB [15] LASIC D, IN PRESS ADV DRUG DE [16] LIGOURE C, 1993, PHYS REV LETT, V71, P3600 [17] LIN A, UNPUB [18] LIVOLANT F, 1989, NATURE, V339, P724 [19] MANNING GS, 1969, J CHEM PHYS, V51, P924 [20] MARSHALL E, 1995, SCIENCE, V269, P1050 [21] MARSHALL E, 1995, SCIENCE, V270, P1751 [22] MULLIGAN RC, 1993, SCIENCE, V260, P926 [23] NABEL GJ, 1993, P NATL ACAD SCI USA, V90, P11307 [24] PERKINS TT, 1994, SCIENCE, V264, P819 [25] PODGORNIK R, 1989, MACROMOLECULES, V22, P1780 [26] RADLER JO, UNPUB [27] REICH Z, 1994, SCIENCE, V264, P1460 [28] ROUX D, 1988, J PHYS-PARIS, V49, P307 [29] SACKMANN E, 1996, SCIENCE, V271, P43 [30] SAFINYA CR, 1986, PHYS REV LETT, V57, P2718 [31] SAFINYA CR, 1989, PHASE TRANSITIONS SO, P249 [32] SELINGER JV, 1991, PHYS REV A, V43, P2922 [33] SINGHAL A, 1994, GENE THERAPEUTICS ME [34] SMITH SB, 1992, SCIENCE, V258, P1122 [35] STERNBERG B, 1994, FEBS LETT, V356, P361 [36] WARRINER HE, 1996, SCIENCE, V271, P969 [37] ZHU N, 1993, SCIENCE, V261, P209 |
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