Single Molecules
Single Molecules |
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| 1 | SINGLE MYOSIN MOLECULE MECHANICS - PICONEWTON FORCES AND NANOMETER STEPS JT. Finer, RM. Simmons, (robert.simmons@tiscali.co.uk )JA. Spudich, (jspudich@stanford.edu ) Nature (1994-03-10) 368-6467 p.113 PubMed Publisher : MACMILLAN PUBLISHERS LTD, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND. ISSN : 0028-0836 Abstract : A new in vitro assay using a feedback enhanced laser trap system allows direct measurement of force and displacement that results from the interaction of a single myosin molecule with a single suspended actin filament. Discrete stepwise movements averaging ai nm were seen under conditions of low load, and single force transients averaging 3-4 pN were measured under isometric conditions. The magnitudes of the single forces and displacements are consistent with predictions of the conventional swinging-crossbridge model of muscle contraction. Corresponding Author : Affiliation(s) : (0) STANFORD UNIV,SCH MED,BECKMAN CTR,DEPT BIOCHEM,STANFORD,CA 94305.; (1) STANFORD UNIV,SCH MED,BECKMAN CTR,DEPT BIOL,STANFORD,CA 94305.; (2) KINGS COLL LONDON,RANDALL INST,MRC MUSCLE & CELL MOTIL UNIT,LONDON WC2B 5RL,ENGLAND.; Key words : RABBIT PSOAS MUSCLE; KINESIN MOLECULES; SLIDING DISTANCE; SKELETAL-MUSCLE; ACTIN FILAMENT; MOVEMENT; INVITRO; FIBERS; CONTRACTION; ATP Type : Article, English. 1994-03-10 Time cited 631; Journal impact factor for year 1994 equals 25.466 [0] BAGSHAW CR, 1993, MUSCLE CONTRACTION, P98 [1] BERGER CL, 1993, BIOCHEMISTRY-US, V32, P3812 [2] BLOCK SM, 1990, NATURE, V348, P348 [3] BRENNER B, 1991, J PHYSIOL-LONDON, V441, P703 [4] BRENNER B, 1991, P NATL ACAD SCI USA, V88, P10490 [5] COOKE R, 1986, CRC CRIT R BIOCHEM, V21, P53 [6] FAJER PG, 1990, P NATL ACAD SCI USA, V87, P5538 [7] FORD LE, 1977, J PHYSIOL-LONDON, V269, P441 [8] FORD LE, 1981, J PHYSIOL-LONDON, V311, P219 [9] GOLDMAN YE, 1977, J PHYSIOL-LONDON, V269, P55 [10] GOLDMAN YE, 1984, J PHYSIOL-LONDON, V354, P577 [11] HARADA Y, 1988, CELL MOTIL CYTOSKEL, V10, P71 [12] HARADA Y, 1990, J MOL BIOL, V216, P49 [13] HASELGROVE JC, 1973, J MOL BIOL, V77, P549 [14] HIGUCHI H, 1991, NATURE, V352, P352 [15] HOWARD J, 1989, NATURE, V342, P154 [16] HUXLEY AF, 1957, PROG BIOPHYS BIOPHYS, V7, P225 [17] HUXLEY AF, 1971, NATURE, V233, P533 [18] HUXLEY HE, 1969, SCIENCE, V164, P1356 [19] HUXLEY HE, 1985, J MUSCLE RES CELL M, V6, P153 [20] ISHIJIMA A, 1991, NATURE, V352, P301 [21] KISHINO A, 1988, NATURE, V334, P74 [22] KRON SJ, 1986, P NATL ACAD SCI USA, V83, P6272 [23] KUO SC, 1993, SCIENCE, V260, P232 [24] LOMBARDI V, 1992, NATURE, V355, P638 [25] MARGOSSIAN SS, 1982, METHOD ENZYMOL, V85, P55 [26] RAYMENT I, 1993, SCIENCE, V261, P50 [27] REEDY MK, 1965, NATURE, V207, P1276 [28] SELLERS JR, 1990, SCIENCE, V249, P406 [29] SIMMONS RM, UNPUB BIOPHYS J [30] SIMMONS RM, 1993, MECHANISM MYOFILAMEN [31] SVOBODA K, IN PRESS REV BIOPHYS [32] SVOBODA K, 1993, NATURE, V365, P721 [33] TAYLOR EW, 1979, CRC CRIT REV BIOCH, V6, P103 [34] TAYLOR EW, 1991, J BIOL CHEM, V266, P294 [35] TOYOSHIMA YY, 1987, NATURE, V328, P536 [36] TOYOSHIMA YY, 1990, P NATL ACAD SCI USA, V87, P7130 [37] UYEDA TQP, 1990, J MOL BIOL, V214, P699 [38] UYEDA TQP, 1991, NATURE, V352, P307 [39] WARREN HM, 1993, MOTILITY ASSAYS MOTO, P1 [40] WHITE HD, 1976, BIOCHEMISTRY-US, V15, P5818 [41] WOLEDGE RC, 1985, ENERGETIC ASPECTS MU, P103 [42] YAMADA A, 1990, J BIOCHEM-TOKYO, V108, P341 [43] YANAGIDA T, 1985, NATURE, V316, P366 [44] YOSHINO S, 1978, BIOPHYS CHEM, V8, P317 |
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